Genome-wide binding analysis of the tomato transcription factor SlDof1 reveals its regulatory impacts on fruit ripening

Abstract The DNA binding with one finger (Dof) proteins are plant-specific transcription factors involved in a variety of biological processes. However, little is known about their functions fruit ripening, flowering-plant-specific process that required for seed maturation and dispersal. Here, we found the tomato Dof factor SlDof1, necessary normal ripening. Knockdown SlDof1 expression by RNA interference delayed ripening-related processes, including lycopene synthesis ethylene production. Transcriptome profiling indicated influences hundreds genes, chromatin immunoprecipitation sequencing revealed large number sites. A total 312 genes were identified as direct targets among which 162 negatively regulated 150 positively regulated. target metabolic pathways, follow-up analyses verified directly regulates some well-known ACS2 PG2A well transcriptional repressor such SlIAA27 . Our findings provide insights into regulatory networks underlying ripening highlight gene potentially useful genetic engineering to control